Trans-splicing and translational control

Polycistronic mRNAs transcribed from operons are resolved into translatable monocistrons by the trans-splicing of a separately transcribed spliced leader (SL) RNA. The SL then forms the 5’ ends of resulting monocistrons. The SL is also trans-spliced to non-operon mRNAs where its function has long remained mysterious. The phylogenetic distribution of trans-splcing is disparate and it … More Trans-splicing and translational control

Transcription initiation codes at developmental transitions

The initiation of transcription at appropriate sites in the genome is critical for development and yet few rules governing selection are known and no core promoter element has so far been assigned a specific role in development. We used cap analysis of gene expression (CAGE) to map transcription start sites (TSSs) at single-bp resolution genome-wide … More Transcription initiation codes at developmental transitions

Recovery from growth arrest

When cultured under crowded and nutrient-limited conditions O. dioica enters a state of growth arrest; halting its reproductive program and extending its lifespan. When returned to favourable conditions animals resume normal development. We tested the transcriptional response to recovery from growth arrest in O. dioica and found that genes encoding monocistronic mRNAs that are not trans-spliced, and encode muscle-related … More Recovery from growth arrest

Chromatin state landscapes

DNA is packaged in the nucleus by winding it around histone proteins to form chromatin. The tails of histone proteins can be covalently modified at a number of specific sites. Specific modifications are associated with open, active chromatin (e.g. acetylations) and others with closed repressed chromatin (e.g. H3K9 methylations). Others are specific to promoters (H3K4me3) … More Chromatin state landscapes